Nuntapramote, Titinun

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Nuntapramote, Titinun
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Nuntapramote, Titinun

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    Item-typ:Veröffentlichung,
    Self-Assembled Fibrinogen Scaffolds Support Cocultivation of Human Dermal Fibroblasts and HaCaT Keratinocytes
    (American Chemical Society, 2023-03-07)
    Joshi, Arundhati
    ;
    Nuntapramote, Titinun
    ;
    Brüggemann, Dorothea
    Self-assembled fibrinogen scaffolds are highly attractive biomaterials to mimic native blood clots. To explore their potential for wound healing, we studied the interaction of cocultures of human dermal fibroblasts (HDFs) and HaCaT keratinocytes with nanofibrous, planar, and physisorbed fibrinogen. Cell viability analysis indicated that the growth of HDFs and HaCaTs was supported by all fibrinogen topographies until 14 days, either in mono- or coculture. Using scanning electron microscopy and cytoskeletal staining, we observed that the native morphology of both cell types was preserved on all topographies. Expression of the marker proteins vimentin and cytokeratin-14 showed that the native phenotype of fibroblasts and undifferentiated keratinocytes, respectively, was maintained. HDFs displayed their characteristic wound healing phenotype, characterized by expression of fibronectin. Finally, to mimic the multilayered microenvironment of skin, we established successive cocultures of both cells, for which we found consistently high metabolic activities. SEM analysis revealed that HaCaTs arranged into a confluent top layer after 14 days, while fluorescent labeling confirmed the presence of both cells in the layered structure after 6 days. In conclusion, all fibrinogen topographies successfully supported the cocultivation of fibroblasts and keratinocytes, with fibrinogen nanofibers being particularly attractive for skin regeneration due to their biomimetic porous architecture and the technical possibility to be detached from an underlying substrate.
    Wissenschaftlicher Artikel
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    Heft:9
      103
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    Organic Electrochemical Transistors and Foreign Body Reaction: First Steps Toward Long-Term Implantable Biosensing
    (Wiley, 2026-03)
    Frulani De Paula Barbosa Henrique
    ;
    Nuntapramote, Titinun
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    Kumar, Ankush
    ;
    Van Den Driesche Sander
    ;
    Vellekoop, Michael J.
    Organic Electrochemical Transistors (OECT) have been widely used to detect a myriad of analytes and signals, ranging from glucose content in sweat to brain epileptiform activity. Due to their biocompatibility, small footprint, conformability, and high signal amplification, OECTs can not only be used in wearable, but also in implanted sensor systems, providing superior signal quality. However, although there is a risk of triggering the Foreign Body Reaction (FBR) with implantation, FBR impact on OECTs has rarely been discussed. Therefore, here we evaluate the effect of the FBR fibrotic response on OECT performance, i.e. when the OECT is covered by protein layers, in vitro. In more detail, we analyze poly(3,4-ethylenedioxythiophene):poly (styrene-sulfonate) (PEDOT:PSS) based OECTs and intentionally cover their channels with protein layers commonly formed during FBR, such as albumin, collagen and fibrinogen. Despite slightly increasing devices' switching time, proteins do not hamper their operation. Further coverage by yeast cells as a proof of concept of wound healing process also did not jeopardize OECT functioning, indicating devices could resist the FBR fibrotic response without anti-FBR strategies.
    Wissenschaftlicher Artikel
    Band:12
    Heft:7
      43
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    Topography-Mediated Induction of Epithelial Mesenchymal Transition via Alumina Textiles for Potential Wound Healing Applications
    (Wiley, 2024-11-11)
    Dutta, Deepanjalee
    ;
    Nuntapramote, Titinun
    ;
    Rehders, Maren
    ;
    Brix, Klaudia
    ;
    Brüggemann, Dorothea
    Substrate topography is vital in determining cell growth and fate of cellular behavior. Although current in vitro studies of the underlying cellular signaling pathways mostly rely on their induction by specific growth factors or chemicals, the influence of substrate topography on specific changes in cells has been explored less often. This study explores the impact of substrate topography, specifically the tricot knit microfibrous structure of alumina textiles, on cell behavior, focusing on fibroblasts and keratinocytes for potential wound healing applications. The textiles, studied for the first time as in vitro substrates, demonstrated support for keratinocyte adhesion, leading to alterations in cell morphology and the expression of E-cadherin and fibronectin. These topography-induced changes resembled the epithelial-to-mesenchymal transition (EMT), crucial for wound healing, and were specific to keratinocytes and absent in identically treated fibroblasts. Biochemically induced EMT in keratinocytes cultured on flat alumina substrates mirrored the changes seen with alumina textiles alone, suggesting the tricot knit microfibrous topography could serve as an in vitro model system to induce EMT-like mechanisms. These results enhance our understanding of how substrate topography influences EMT-related processes in wound healing, paving the way for further evaluation of microfibrous alumina textiles as innovative wound dressings.
    Wissenschaftlicher Artikel
      101