Dierker, Lea

Lade...
Vorschaubild
Preferred name
Dierker, Lea
Official Name
Dierker, Lea

Filter

2024 - 20252

Einstellungen

Gerade angezeigt 1 - 2 von 2
  • Lade...
    Vorschaubild
    Some of the metrics are blocked by your 
    consent settings
    Item-typ:Veröffentlichung,
    Structure, Properties and Degradation of Self-Assembled Fibrinogen Nanofiber Scaffolds
    (American chemical society, 2024-09-16)
    Strunk, Till
    ;
    Joshi, Arundhati
    ;
    Moeinkhah, Mahta
    ;
    Renzelmann, Timon
    ;
    Dierker, Lea
    Self-assembled fibrinogen nanofibers are promising candidates for skin tissue engineering due to their biocompatibility and ability to mimic the native blood clot architecture. Here, we studied the structure-property relationship and degradation of rehydrated fibrinogen nanofibers prepared by salt-induced self-assembly, focusing on the effect of scaffold layering, cross-linking time and freeze-drying. Optimal fiber stability was achieved with cross-linking by formaldehyde (FA) vapor, while treatment with liquid aldehydes, genipin, EDC, and transglutaminase failed to preserve the nanofibrous architecture upon rehydration. Scaffold layering did not significantly influence the mechanical properties but changed the scaffold architecture, with bulk fiber scaffolds being more compact than layered scaffolds. Freeze-drying maintained the mechanical properties and interconnected pore network with average pore diameters around 20 μm, which will enhance the storage stability of self-assembled fibrinogen scaffolds. Varying cross-linking times altered the scaffold mechanics without affecting the swelling behavior, indicating that scaffold hydration can be controlled independently of the mechanical characteristics. Cross-linking times of 240 min increased scaffold stiffness and decreased elongation, while 30 min resulted in mechanical properties similar to native skin. Cross-linking for 120 min was found to reduce scaffold degradation by various enzymes in comparison to 60 min. Overall, after 35 days of incubation, plasmin and a combination of urokinase and plasminogen exhibited the strongest degradative effect, with nanofibers being more susceptible to enzymatic degradation than planar fibrinogen due to their higher specific surface area. Based on these results, self-assembled fibrinogen fiber scaffolds show great potential for future applications in soft tissue engineering that require controlled structure-function relationships and degradation characteristics.
    Wissenschaftlicher Artikel
    Band:7
    Heft:9
      87
  • Lade...
    Vorschaubild
    Some of the metrics are blocked by your 
    consent settings
    Item-typ:Veröffentlichung,
    Advancing Research on Biomaterials and Biological Materials with Scanning Electron Microscopy under Environmental and Low Vacuum Conditions
    (Wiley, 2025-12)
    Riedel, Jendrian
    ;
    Dierker, Lea
    ;
    Kwame, Antoine Eyram
    ;
    Wagner, Kim Nora
    ;
    Unterholzner Jonas J
    In this article, a general introduction to the fundamental principles of environmental scanning electron microscopy (ESEM) is given and its advantages in comparison to conventional SEM is illustrated through selected application examples from life sciences, focusing on biomaterial research and biology. Conventional SEM is a well-established and widely used technique to characterize material properties and surface structures in a variety of research fields. However, due to the high vacuum conditions used in conventional SEM, imaging delicate, nonconductive, and/or wet samples—which are particularly prevalent in life sciences—normally requires extensive sample preparation, such as critical point drying and sputter-coating, which can alter the sample properties. Here, ESEM offers a convenient solution, where nonconductive and wet biological samples can be imaged in their near-native state almost without sample preparation. The advantages of ESEM compared to conventional SEM for life sciences based on examples from literature and new application examples from biomaterials science and zoology are illustrated. Finally, recent advances in automated imaging and AI-based image processing are beginning to extend the current limits of resolution and sample stability in ESEM, potentially enabling more precise, real-time, and less damaging imaging of hydrated and beam-sensitive materials in future studies.
    Wissenschaftlicher Artikel
      96