Ionentransporte in C6-Glioma-Zellen bei oxidativem Streß

Abstract

In this work the dose dependent disorders of ion transport processes and mitochondrial membrane potential caused by hydrogen peroxide were investigated with fluorescencespectroscopic methods in C6-Glioma-Cells. These cells served as a model system for one glial subtype, the astrocytes. The transition pore is known to precede the programmed cell death, apoptosis. The apoptotic pathway is interrupted if all mitochondria of the affected cell undergo depolarisation. The following deficiency of high energy substrates leads to cell swelling and plasmamembrane disrupture, the so called onkosis. The results of this work indicate that the mitochondria are involved in the signal cascade of the oxidative stress response in C6-Glioma-Cells.