Gerlach, Nadine
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Gerlach, Nadine
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Gerlach, Nadine
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Item-typ:Veröffentlichung, Bacterial utilization of anionic polysaccharides from macroalgae(2020-10-08); ; ; About two thirds of Earth is covered by oceans of which less than 2 % constitute marine vegetation. Yet, marine vegetation serves crucial ecosystem functioning and is especially vital to the health of coastal ecosystems. Macroalgae display one group of marine vegetation which can form ecological habitats that are not only impressive by their dimensions but also with regards to biodiversity and primary production. Among algal compounds, polysaccharides play an important role as bioactive polymers and food source for heterotrophic organisms. Especially anionic polysaccharides are of high scientific interest as they define the 3D structure of the algae and sequester carbon in form of sinking particles from the photic zone to the deep sea. Detailed insights into the enzymatic machinery of heterotrophic bacteria capable to utilize algal polysaccharides contributes to a better understanding of carbon cycling and food web dynamics in the marine environment. In the present dissertation, I investigated different aspects of bacterial utilization of three anionic polysaccharides from macroalgae – ulvan, alginate and fucoidan. The research in the present dissertation delivered molecular insights into the function and structure of glycan-degrading enzymes. These findings will contribute to a greater understanding of the utilization of anionic polysaccharides by marine heterotrophic bacteria. Consequently, using bacterial enzymes as analytical tools to investigate the turnover rates of anionic polysaccharides will elucidate their fate in the marine carbon cycle.Dissertation254 192 - Some of the metrics are blocked by yourconsent settings
Item-typ:Veröffentlichung, Biochemical and structural analysis of marine polysaccharide lyases(2017-05-24); This study investigated marine bacterial PLs capable of degrading the anionic algal polysaccharides alginate and ulvan, respectively. A particular focus was given to (i) an alginolytic system in Alteromonas macleodii 83-1 and (ii) a putative polysaccharide utilization locus for ulvan in Formosa agariphila KMM3901 . The alginolytic system in Alteromonas macleodii 83-1 was studied by producing its five Alys as recombinant proteins, which were expressed in E. coli, and purified via chromatography in order to investigate enzyme activity and kinetics. In addition, phylogenetic analysis was done as well as protein crystallization to solve the enzyme structure of PL7-1. This project is part of the work of Dr. Wietz who is doing culture-based and genomic studies on Alteromonas macleodii 83-1. The analysis of a polysaccharide utilization locus for ulvan in Formosa agariphila T KMM3901 is part of collaboration between the MPI Bremen and the University of Greifswald. Our colleagues from Greifswald are currently working on enzyme kinetics and activity, whereas we contributed the structural analysis of an undescribed ulvan lyase. Our work included protein purification and analytical chromatography, determination of thermal stability, and X-ray crystallography. The present study provides detailed insights into the biochemistry, evolution and functionality of PLs from F. agariphila and A. macleodii with focus on PL7-1 (alginate), as well as PL24 and PL25 (ulvan). Studying the utilization pathways of marine heterotrophic bacteria capable of degrading reduced carbon compounds such as algal polysaccharides contributes to the understanding of processes that considerably impact marine food web dynamics and carbon cycling in the oceans.Masterarbeit404 271