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  4. Intact polar lipids in marine sediments : improving analytical protocols and assessing planktonic and benthic sources
 
Zitierlink URN
https://nbn-resolving.de/urn:nbn:de:gbv:46-00104913-12

Intact polar lipids in marine sediments : improving analytical protocols and assessing planktonic and benthic sources

Veröffentlichungsdatum
2015-11-26
Autoren
Schröder, Jan Martin  
Betreuer
Hinrichs, Kai-Uwe  
Gutachter
Zabel, Matthias  
Zusammenfassung
This PhD thesis focused on the analysis and application of microbial membrane lipids as biomarkers in marine sediments. Existing protocols for lipid extraction from marine sediments and biomass were modified. In addition, recent protocols based on high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS) as well as state-of-the-art mass spectrometric analysis by quadrupole time-of-flight (MSqTOF) and the triple quadrupole (MSQQQ) mass spectrometer were used to investigate matrix effects and evaluate the reliability of quantitative analysis in marine environmental samples. The improved lipid extraction and quantification were used to analyze Black Sea water column and sediments samples to a depth of 8 meters from site GeoB 15105 taken during cruise M84/1 (DARCSEAS) with R/V Meteor to apply lipid analysis in benthic bio systems. With this component specific differentiation between planktonic and benthic lipid signature we assessed possible lipid sources. Here, this high detail lipid fingerprinting allowed us to observe changes in the head group and lipid core structures of the intact polar lipids according to the geochemical zonation.
Schlagwörter
Intact polar lipids

; 

IPL

; 

lipid biomarker

; 

Black Sea

; 

marine sediment

; 

lipid analysis

; 

matrix effect

; 

response factor

; 

quantitative lipid analysis

; 

Archaea

; 

Bacteria

; 

HPLC-MS

; 

deep biosphere

; 

GDGT

; 

archaeol
Institution
Universität Bremen  
Fachbereich
Fachbereich 05: Geowissenschaften (FB 05)  
Dokumenttyp
Dissertation
Zweitveröffentlichung
Nein
Sprache
Englisch
Dateien
Lade...
Vorschaubild
Name

00104913-1.pdf

Size

34.83 MB

Format

Adobe PDF

Checksum

(MD5):3496357ee98086c2926c7260bbd3a483

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