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Citation link: https://doi.org/10.26092/elib/3200

Publisher DOI: https://doi.org/10.1021/acsabm.9b00415
Reshma_Maas_Rezwan_Selective, Agglomerate-Free Separation of Bacteria Using Biofunctionalized, Magnetic Janus Nanoparticles_2019_submitted-version.pdf
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Selective, Agglomerate-Free Separation of Bacteria Using Biofunctionalized, Magnetic Janus Nanoparticles


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Reshma_Maas_Rezwan_Selective, Agglomerate-Free Separation of Bacteria Using Biofunctionalized, Magnetic Janus Nanoparticles_2019_submitted-version.pdf2.37 MBAdobe PDFView/Open
Authors: Kadam, Reshma Sampat  
Maas, Michael  
Rezwan, Kurosch  
Abstract: 
This study presents a scalable method for designing magnetic Janus nanoparticles, which are capable of performing bacterial capture, while preventing agglomeration between bacterial cells. To this end, we prepared silica-coated magnetite Janus nanoparticles functionalized with a bacteria-specific antibody on one side and polyethylene glycol chains on the other, using the established wax-in-water emulsion strategy. These magnetic Janus nanoparticles specifically interact with one type of bacteria from a mixture of bacteria via specific antigen–antibody interactions. Contrarily to bacterial capture with isotropically functionalized particles, the bacterial suspensions remain free from cell–nanoparticle–cell agglomerates, owing to the passivation coating with polyethylene glycol chains attached to the half of the magnetic nanoparticles pointing away from the bacterial surface after capture. The selective magnetic capture of Escherichia coli cells was achieved from a mixture with Staphylococcus simulans without compromising bacterial viability and with an efficiency over 80%. This approach is a promising method for rapid and agglomeration-free separation of live bacteria for identification, enrichment, and cell counting of bacteria from biological samples.
Keywords: Janus; Nanoparticles; Nonagglomerating; E. coli; Separation; Peptides and proteins
Issue Date: 10-Jul-2019
Publisher: American Chemical Society Publications
Journal/Edited collection: ACS Applied Bio Materials 
Issue: 8
Start page: 3520
End page: 3531
Volume: 2
Type: Artikel/Aufsatz
ISSN: 2576-6422
Secondary publication: yes
Document version: Preprint
DOI: 10.26092/elib/3200
URN: urn:nbn:de:gbv:46-elib81661
Institution: Universität Bremen 
Faculty: MAPEX Center for Materials and Processes 
Fachbereich 05: Geowissenschaften (FB 05) 
Appears in Collections:Forschungsdokumente

  

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