Embedding live bacteria in porous hydrogel/ceramic nanocomposites for bioprocessing applications
Veröffentlichungsdatum
2019-04-05
Zusammenfassung
In this work, we present a biocompatible one-pot processing route for ceramic/hydrogel nanocomposites in which we embed live bacteria. In our approach, we fabricate a highly stable alginate hydrogel with minimal shrinkage, highly increased structural and mechanical stability, as well as excellent biocompatibility. The hydrogel was produced by ionotropic gelation and reinforced with alumina nanoparticles to form a porous 3D network. In these composite gels, the bacteria Escherichia coli and Bacillus subtilis were embedded. The immobilized bacteria showed high viability and similar metabolic activity as non-embedded cells. Even after repeated glucose consumption cycles, the material maintained high structural stability with stable metabolic activity of the immobilized bacteria. Storing the bionanocomposite for up to 60 days resulted in only minor loss of activity. Accordingly, this approach shows great potential for producing macroscopic bioactive materials for biotechnological processes.
Schlagwörter
Cell encapsulation
;
Ceramic nanoparticles
;
Hydrogel
;
nanocomposite materials
;
Mimenima
Verlag
Springer
Institution
Dokumenttyp
Artikel/Aufsatz
Zeitschrift/Sammelwerk
Band
42
Startseite
1215
Endseite
1224
Zweitveröffentlichung
Ja
Dokumentversion
Postprint
Lizenz
Sprache
Englisch
Dateien![Vorschaubild]()
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Name
Condi_Embedding live bacteria in porous hydrogel ceramic nanocomposites_accepted version_Deckblatt_pdfa1.pdf
Size
1.77 MB
Format
Adobe PDF
Checksum
(MD5):3bdb4ba1900bc565c1f6ff48d69de408