Microparticle trajectories in a high-throughput channel for contact-free fractionation by dielectrophoresis
Veröffentlichungsdatum
2016-07-22
Zusammenfassung
Continuous, contact-free fractionation of sensitive microparticles at high throughput is a challenge. For this purpose, we developed a sheath flow assisted dielectrophoretic (DEP) field-flow separator with a tailored arrangement of cylindrical interdigitated electrodes (cIDE) and observed size-dependent trajectories of dispersed particles. Using a voltage input of 200 Veff at a frequency of 200 kHz, polystyrene particles (45, 25, and 11 µm in diameter) levitated to different heights along the channel length due to a negative DEP force. Experimental observations agree well with simulated particle trajectories that were obtained by a modified Lagrangian particle tracking model in combination with Laplace's and Navier–Stokes equations. By exploiting the size-dependent levitation height difference the desired particle size fraction can be collected at a specific channel length. The required channel length of the proposed cIDE separator increases with decreasing particle size to be separated. The quality of theoretical fractionation, which we quantify by resolution, improves strongly with reduced collector width, reduced volume flow rate and increased voltage input. The sensitivity of these dependencies increases with decreasing particle size. We calculated a theoretical throughput of up to 47 mL min−1 when trading-off design and operation parameters, allowing for contact-free fractionation of sensitive microparticles with negligible shear stress.
Schlagwörter
Fractionation
;
Dielectrophoresis
;
Particle trajectory
;
Resolution
;
High throughput
;
Mimenima
Verlag
Elsevier
Institution
Dokumenttyp
Artikel/Aufsatz
Zeitschrift/Sammelwerk
Band
153
Startseite
34
Endseite
44
Zweitveröffentlichung
Ja
Dokumentversion
Postprint
Sprache
Englisch
Dateien![Vorschaubild]()
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Name
Y. Wang, F. Du, G.R. Pesch, J. Köser, M. Baune, J.Thöming, Microparticle trajectories in a high-throughput channel_2016_accepted version_Deckblatt_pdfa1.pdf
Size
1.36 MB
Format
Adobe PDF
Checksum
(MD5):de3c3609a49da6508442a6ccad69d884