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Citation link: https://media.suub.uni-bremen.de/handle/elib/6492

Publisher DOI: https://doi.org/10.1016/j.msec.2021.112156
 
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Self-assembled fibrinogen nanofibers support fibroblast adhesion and prevent E. coli infiltration


Authors: Suter, Naiana  
Joshi, Arundhati 
Wunsch, Timo 
Graupner, Nina  
Stapelfeldt, Karsten  
Radmacher, Manfred  
Müssig, Jörg  
Brüggemann, Dorothea  
Abstract: 
Fibrinogen nanofibers hold great potential for wound healing applications since they mimic the native blood clot architecture and offer important binding sites to support fibroblast adhesion and migration. Recently, we introduced a new method of saltinduced self-assembly to prepare nanofibrous fibrinogen scaffolds. Here, we present our results on the mechanical properties of these scaffolds and their interaction with 3T3 fibroblasts and E. coli bacteria, which we used as model systems for wound healing. Hydrated, nanofibrous fibrinogen scaffolds showed a Young's modulus of 1.3 MPa, which is close to the range of native fibrin. 3T3 fibroblasts adhered and proliferated well on nanofibrous and planar fibrinogen up to 72 h with a less pronounced actin cytoskeleton on nanofibers in comparison to planar fibrinogen. Fibroblasts on nanofibers were smaller with many short filopodia while larger cells with few long filopodia were found on planar fibrinogen. Live cell tracking revealed higher migration velocities on nanofibers in comparison to planar fibrinogen. The growth of E. coli bacteria on nanofibrous fibrinogen was significantly reduced as compared to agar controls with no bacteria migrating through the nanofibers. In summary, we conclude that self-assembled fibrinogen nanofibers could become highly attractive as future scaffolds for wound healing applications.
Keywords: 3D cell culture; bacteria; Nanotopography; Protein pattern; Self-assembly; Tissue engineering; Wound healing
Issue Date: Jul-2021
Publisher: Elsevier Science
Journal/Edited collection: Materials science & engineering 
Start page: 112156
Note: 126
Type: Artikel/Aufsatz
ISSN: 09284931
Institution: Hochschule Bremen 
Faculty: Hochschule Bremen - Fakultät 5: Natur und Technik 
Appears in Collections:Bibliographie HS Bremen

  

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