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Publisher DOI: https://doi.org/10.1038/s41556-021-00767-x
https://media.suub.uni-bremen.de/handle/elib/6449
Publisher DOI: https://doi.org/10.1038/s41556-021-00767-x
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Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
Authors: | Veltel, Stefan Moreno-Layseca, Paulina Jäntti, Niklas Godbole, Rashmi Sommer, Christian Jacquemet, Guillaume Al-Akhrass, Hussein Conway, James R. W. Kronqvist, Pauliina Kallionpää, Roosa E. Oliveira-Ferrer, Leticia Cervero, Pasquale Linder, Stefan Aepfelbacher, Martin Zauber, Henrik Rae, James Parton, Robert G. Disanza, Andrea Scita, Giorgio Mayor, Satyajit Selbach, Matthias Ivaska, Johanna |
Abstract: | Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery—Arf1, IRSp53 and actin—and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route. |
Keywords: | breast cancer; cell adhesion; endosomes | Issue Date: | 2021 | Publisher: | Nature Publ. Group. | Journal/Edited collection: | Nature Cell Biology | Start page: | 1073 | End page: | 1084 | Note: | 23 | Type: | Artikel/Aufsatz | ISSN: | 1465-7392 | Institution: | Hochschule Bremen | Faculty: | Hochschule Bremen - Fakultät 5: Natur und Technik |
Appears in Collections: | Bibliographie HS Bremen |
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