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  4. Semi-automated differentiation of insect exo- and endocuticle in X-ray microtomography
 
Verlagslink DOI
10.1016/j.asd.2021.101139

Semi-automated differentiation of insect exo- and endocuticle in X-ray microtomography

Veröffentlichungsdatum
2022-01
Autoren
Stamm, Karen  
Dirks, Jan-Henning  
Zusammenfassung
One of the most versatile and complex biological composite materials, the insect exoskeleton shows a huge range in biomechanical properties. The cuticle exoskeleton can be differentiated into two main histologically different layers with distinct properties: the outer, more sclerotized exocuticle and inner, softer endocuticle. For most biomechanical research questions, it is of great importance to be able to selectively characterize geometrical features of these layers. However, most conventional preparation methods (cross-sections, histological staining, SEM) require complex and destructive sample preparation, which provides only two-dimensional information. Here, we present a novel, simple staining method using X-ray microtomography to distinguish between exo- and endocuticle in a 3D environment without sample destruction. We illustrate the power of our method using locust (Locusta migratoria) hindleg tibia, a well characterized biomechanical sample. Our method allows an easy and direct measurement of exo- and endocuticle and their respective geometric features. Applying our method will help to understand the biomechanical role of exo- and endocuticle within an insect exoskeleton and will allow us to understand its composition and morphological features in more detail.
Schlagwörter
cuticle layer differentiation

; 

Insect exoskeleton

; 

novel staining method

; 

X-ray microtomography
Verlag
Elsevier {BV}
Institution
Hochschule Bremen  
Fachbereich
Hochschule Bremen - Fakultät 5: Natur und Technik  
Dokumenttyp
Artikel/Aufsatz
Zeitschrift/Sammelwerk
Arthropod structure & development  
Band
66
Zweitveröffentlichung
Nein
Lizenz
Alle Rechte vorbehalten
Sprache
Englisch

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