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Citation link: http://nbn-resolving.de/urn:nbn:de:gbv:46-00103275-17
00103275-1.pdf
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Identification of the Molecular Mechanisms underlying the Gastrointestinal Toxicity of the Immunosuppressants Mycophenolate Mofetil and Enteric-coated Mycophenolate Sodium using a Proteo-Metabolomic Approach


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Other Titles: Identifizierung der molekularen Mechanismen der gastrointestinalen Toxizität der Immunsuppressiva Mycophenolat-Mofetil und magensaftresistentem Mycophenolat-Natrium durch kombinierte Proteom- und Metabolomanalysen
Authors: Heischmann, Svenja 
Supervisor: Christians, Uwe 
1. Expert: Leibfritz, Dieter
2. Expert: Stohrer, Wolf-Dieter
Abstract: 
Mycophenolic acid (MPA) is the cornerstone of most immunosuppressive drug regimens after organ transplantation. However, gastrointestinal side effects diminish MPA's otherwise outstanding safety and efficiency. Using a unique cell culture model featuring the human colon cancer cell line LS180 and a combination of molecular biology methods and a non-targeted proteo-metabolomic strategy based on high-performance liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy, we were able to identify impairment of LS180 cell lipid metabolism as well as effects on structural proteins and nucleotide-dependent processes as main mediators of the negative effects of MPA on gastrointestinal barrier function.
Keywords: Mycophenolic acid, gastrointestinal toxicity, LS180 cells, HPLC-MS, NMR, proteomics, metabolomics
Issue Date: 11-Jun-2013
Type: Dissertation
URN: urn:nbn:de:gbv:46-00103275-17
Institution: Universität Bremen 
Faculty: FB2 Biologie/Chemie 
Appears in Collections:Dissertationen

  

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