Analysis of the Genome Stability of Uterine Fibroids with Different Degree of HMGA Expression

Abstract

Uterine leiomyomas (UL) are attended scientifically due to their high prevalence, irrecoverable complications, and high therapeutic burden. Genetic factors are among the suggested factors for the initiation of UL. This thesis aims at the cytogenetic molecular investigations in a large series of UL, to find the correlation between chromosomal changes and the expression of related genes, as well as the genome instability in correlation with these genes. This study has discovered: Karyotype abnormality in 29.1% of UL Overexpression of HMGA1 in the myomas with 6p21 aberrations than that in myometrium and normal UL Overexpression of HMGA2 in the UL with 12q14~15 rearrangements than that in matched myometrium and normal UL Overexpression of HMGA2 also in normal UL compared with myometrial tissue Much less strong increase of the average expression of HMGA1 in UL with 6p21 rearrangements than that of HMGA2 in case of 12q14~15 aberrations No immortalization of myoma cells No correlation between HMGA2 level and the increase of genomic instability