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  4. Expression und Charakterisierung des Andes-Virus L-Proteins
 
Zitierlink URN
https://nbn-resolving.de/urn:nbn:de:gbv:46-00103254-15

Expression und Charakterisierung des Andes-Virus L-Proteins

Veröffentlichungsdatum
2013-05-31
Autoren
Heinemann, Patrick  
Betreuer
Schmidt-Chanasit, Jonas  
Gutachter
Günther, Stephan  
Zusammenfassung
Andes virus (ANDV) is a member of the genus Hantavirus. Little is known about structure and function of the hantaviral L protein. Sequence alignments revealed a putative RNA-dependent RNA polymerase domain in the center and an endonuclease at the N terminus. Studying hantaviral L proteins is difficult, because recombinant L protein expression in mammalian cells is very ineffective. Deletion analysis revealed that the N terminus determine the low-expression phenotype. This work demonstrates that ANDV L protein expression can be rescued upon mutation of catalytic amino acids and further conserved residues of the putative endonuclease domain. In addition, wild-type ANDV L rather than expressible L mutants suppressed the level of mRNA and reduces protein expression. Expressible ANDV L mutants colocalized with the cellular processing (P) bodies, ANDV N and NSs protein, but not with the glycoprotein Gc. This work suggests that ANDV L protein possesses an endonuclease at the N terminus suppressing the level of its own as well as heterologous mRNAs upon recombinant expression in mammalian cells.
Schlagwörter
Hantavirus

; 

polymerase

; 

L protein

; 

endonuclease
Institution
Universität Bremen  
Fachbereich
Fachbereich 02: Biologie/Chemie (FB 02)  
Dokumenttyp
Dissertation
Zweitveröffentlichung
Nein
Sprache
Deutsch
Dateien
Lade...
Vorschaubild
Name

00103254-1.pdf

Size

8.31 MB

Format

Adobe PDF

Checksum

(MD5):588fea1eb084e50f8133e64c5dc8b413

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