Investigation of the role of class I KNOX genes in the floral development of Eschscholzia californica
|Other Titles:||Untersuchung der Rolle von Klasse I KNOX Genen in der Blütenentwicklung bei Eschscholzia californica||Authors:||Stammler, Angelika||Supervisor:||Koenig, Friederike||1. Expert:||Koenig, Friederike||2. Expert:||Blohm, Dietmar||Abstract:||
Four class I KNOX genes were isolated from the Eschscholzia californica, Papaveraceae. Phylogenetic analysis suggests that these four KNOX genes cover all three for the model Arabidopsis thaliana established clades of class I KNOX genes, STM (SHOOT APICAL MERISTEM-LESS), KNAT1/BP (Knotted-like Arabidopsis thaliana-1/BREVIPEDICELLUS) and KNAT2/6 (Knotted-like Arabidopsis thaliana-2/6). Two of these genes (EcSTM1 and EcSTM2) were identified as STM-like genes. Expression analysis in vegetative and floral shoots tips revealed a continuous expression of EcSTM1, EcKNAT1 and EcKNAT2, whereas EcSTM2 is exclusively expressed in early floral buds. EcSTM1-VIGS (Virus-induced gene silencing) resulted in the absence of the inner floral whorls. In flowers of EcSTM2-VIGS treated plants, the carpels were initiated but did not develop properly. Rarely, a floral reversion was observed. Combined silencing of EcSTM1 and EcSTM2 led to combinations of the phenotypes observed for EcSTM1-VIGS and EcSTM2-VIGS.
|Keywords:||carpel, gynoecium, KNOX, STM, Eschscholzia, Papaveraceae||Issue Date:||9-Aug-2010||URN:||urn:nbn:de:gbv:46-00101746-17||Institution:||Universität Bremen||Faculty:||FB2 Biologie/Chemie|
|Appears in Collections:||Dissertationen|
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