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Citation link: http://nbn-resolving.de/urn:nbn:de:gbv:46-diss000118430
00011843.pdf
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Untersuchungen zum Stoffwechsel des anaeroben Alkanabbaus


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Other Titles: Study of anaerobic alkane metabolism
Authors: Werner, Insa 
Supervisor: Widdel, Friedrich
1. Expert: Widdel, Friedrich
2. Expert: Harder, Jens
Abstract: 
Strain HxN1, an Azoarcus-like denitrifying bacterium, degrades the saturated hydrocarbon n-hexane under anoxic conditions. Former studies on metabolites formed during the anaerobic degradation of n-alkanes suggested an activation mechanism analogous to that in the anaerobic degradation of toluene. The postulated enzyme (Mas, methylpentylsuccinate-synthase) is expected to belong to the class of glycyl radical enzymes. It was biochemically characterized by an enzymatic study using cell-free extract of strain HxN1. Furthermore, the enzyme was successfully purified under strictly anoxic conditions proofing the predicted function of the enzyme. A fourth by then unknown subunit of the enzyme was identified. Homologues of this subunit could be found in other n-alkane degrading organisms as well. Another part focused on the relationship of different mas-genes. In three strains tentative n-alkane activating genes could be identified strongly related to methylpentylsuccinate-synthase.
Keywords: anaerobic alkane degradation, glycyl radical enzyme
Issue Date: 15-Dec-2009
Type: Dissertation
URN: urn:nbn:de:gbv:46-diss000118430
Institution: Universität Bremen 
Faculty: FB2 Biologie/Chemie 
Appears in Collections:Dissertationen

  

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