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Untersuchungen zum Stoffwechsel des anaeroben Alkanabbaus
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Other Titles: | Study of anaerobic alkane metabolism | Authors: | Werner, Insa | Supervisor: | Widdel, Friedrich | 1. Expert: | Widdel, Friedrich | 2. Expert: | Harder, Jens | Abstract: | Strain HxN1, an Azoarcus-like denitrifying bacterium, degrades the saturated hydrocarbon n-hexane under anoxic conditions. Former studies on metabolites formed during the anaerobic degradation of n-alkanes suggested an activation mechanism analogous to that in the anaerobic degradation of toluene. The postulated enzyme (Mas, methylpentylsuccinate-synthase) is expected to belong to the class of glycyl radical enzymes. It was biochemically characterized by an enzymatic study using cell-free extract of strain HxN1. Furthermore, the enzyme was successfully purified under strictly anoxic conditions proofing the predicted function of the enzyme. A fourth by then unknown subunit of the enzyme was identified. Homologues of this subunit could be found in other n-alkane degrading organisms as well. Another part focused on the relationship of different mas-genes. In three strains tentative n-alkane activating genes could be identified strongly related to methylpentylsuccinate-synthase. |
Keywords: | anaerobic alkane degradation, glycyl radical enzyme | Issue Date: | 15-Dec-2009 | Type: | Dissertation | URN: | urn:nbn:de:gbv:46-diss000118430 | Institution: | Universität Bremen | Faculty: | FB2 Biologie/Chemie |
Appears in Collections: | Dissertationen |
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