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Zitierlink URN
https://nbn-resolving.de/urn:nbn:de:gbv:46-diss000118430

Untersuchungen zum Stoffwechsel des anaeroben Alkanabbaus

Veröffentlichungsdatum
2009-12-15
Autoren
Werner, Insa  
Betreuer
Widdel, Friedrich  
Gutachter
Harder, Jens  
Zusammenfassung
Strain HxN1, an Azoarcus-like denitrifying bacterium, degrades the saturated hydrocarbon n-hexane under anoxic conditions. Former studies on metabolites formed during the anaerobic degradation of n-alkanes suggested an activation mechanism analogous to that in the anaerobic degradation of toluene. The postulated enzyme (Mas, methylpentylsuccinate-synthase) is expected to belong to the class of glycyl radical enzymes. It was biochemically characterized by an enzymatic study using cell-free extract of strain HxN1. Furthermore, the enzyme was successfully purified under strictly anoxic conditions proofing the predicted function of the enzyme. A fourth by then unknown subunit of the enzyme was identified. Homologues of this subunit could be found in other n-alkane degrading organisms as well. Another part focused on the relationship of different mas-genes. In three strains tentative n-alkane activating genes could be identified strongly related to methylpentylsuccinate-synthase.
Schlagwörter
anaerobic alkane degradation

; 

glycyl radical enzyme
Institution
Universität Bremen  
Fachbereich
Fachbereich 02: Biologie/Chemie (FB 02)  
Dokumenttyp
Dissertation
Zweitveröffentlichung
Nein
Sprache
Deutsch
Dateien
Lade...
Vorschaubild
Name

00011843.pdf

Size

1.7 MB

Format

Adobe PDF

Checksum

(MD5):2a7afd2c693ae44edb9b8b03a75fe6a2

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