New organic substrates for anoxygenic phototrophic bacteria
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Other Titles: | Neue organische Substrate für anoxigene phototrophe Bakterien | Authors: | Tran, Nhu Hoa | Supervisor: | Widdel, Friedrich | 1. Expert: | Widdel, Friedrich | Experts: | Harder, Jens | Abstract: | For the first time, a purple sulfur bacterium (strain E3P) able to utilize AH2QDS and reduced humic acids was isolated. 16S rRNA gene sequence of strain E3P showed the closest relative with Thiocystis violacea (95.8%). AQDS and humic acids were also shown to serve as electron shuttles between the chemotrophic Geobacter metallireducens and strain E3P. Enrichments from marine sediments with n-hexane and n-decane as substrates for anoxygenic phototrophic bacteria were established. Analysis of the enriched microbial communities by DGGE revealed phylotypes closely related to described anoxygenic phototrophic and sulfate-reducing bacteria. Results suggest that sulfate-reducing bacteria in the enrichment culture coupled the degradation of n-hexane or n-decane to the reduction of sulfate to sulfide. The latter could be used by phototrophic bacteria.A phototrophic strain utilizing toluene was isolated (strain GH1). This strain shared 99% 16S rRNA gene sequence similarity and 92.7% DNA-DNA similarity with a described toluene-utilizing phototrophic bacterium, Blastochloris sulfoviridis strain ToP1. In both strains, the gene bssA was detected. |
Keywords: | Anoxygenic phototrophic bacteria; humic acids; AH2QDS; electron shuttle; hydrocarbons | Issue Date: | 24-Apr-2008 | Type: | Dissertation | Secondary publication: | no | URN: | urn:nbn:de:gbv:46-diss000109785 | Institution: | Universität Bremen | Faculty: | Fachbereich 02: Biologie/Chemie (FB 02) |
Appears in Collections: | Dissertationen |
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