Zelluläre und subzelluläre Testsysteme zur Beurteilung des biologischen Gefährdungspotenzials von Chemikalien im nachhaltigen Chemikaliendesign.Konzept einer Teststrategie und ihre Anwendung in zwei Fallbeispielen

Abstract

Green chemicals should display no or little toxicity for men and environment. But how can it be guaranteed that new chemicals meet these requirements? A concept was developed to assess in a quick and easy way the hazard potential of a chemical already during the design of this chemical. This assessment is based on cellular and sub-cellular test systems. In a first step the overall potential of a set of chemicals to interact with biological structures is assessed based on the T-SAR concept (thinking in terms of structure-activity relationships) and on tests with cell cultures. In a second step, specific interaction potentials are assessed. Based on the results of the first step a set of sub cellular test systems for specific interactions with biological structures such as enzyme inhibition is selected (flexible test battery). Results of both steps are included in a hazard potential profile. The concept was applied for two case studies using two biocides and a set of ionic liquids. Results show, that the concept allows a first assessment of the hazard potential of a chemical. However further test systems for specific interaction potentials need to be included.