Skip navigation
SuUB logo
DSpace logo

  • Home
  • Institutions
    • University of Bremen
    • City University of Applied Sciences
    • Bremerhaven University of Applied Sciences
  • Sign on to:
    • My Media
    • Receive email
      updates
    • Edit Account details

Citation link: http://nbn-resolving.de/urn:nbn:de:gbv:46-diss000106092
00010609.pdf
OpenAccess
 
copyright

Untersuchungen zu den Regulationsmechanismen der Typ IV-Pilingene aus Azoarcus sp. BH72 und der Involvierung von Quorum Sensing


File Description SizeFormat
00010609.pdf3.57 MBAdobe PDFView/Open
Other Titles: Investigations concerning the regulation mechanisms of the type IV pilin genes of Azoarcus sp. BH72 and the involvement of quorum sensing
Authors: Böhm, Melanie 
Supervisor: Reinhold-Hurek, Barbara
1. Expert: Reinhold-Hurek, Barbara
2. Expert: Koenig, Friederike
Abstract: 
Azoarcus sp. BH72 is a diazotrophic grass endophyte. The interaction with the rice plant is mediated by the type IV pilus, which is encoded by the pilAB operon. In the genome sequence pilT was identified, which encodes for a putative type IV pilus retraction protein. To gain more insights into the role of type IV pili during the colonization process, the pilT and pilA genes were inactivated by mutagenesis. Both mutants were abolished in twitching motility. Enhanced piliation of the pilT mutant indicates that indeed PilT has a role in pilus retraction. Studies on rice root colonization revealed that the retractile force mediated by PilT is not essential for the bacterial colonization of the plant surface, but that twitching motility is necessary for invasion and establishment inside the plant.Monitoring the pilAB expression with the reporter genes gusA and gfp at various time points revealed an induction of the pilAB gene expression in a cell density dependent manner. Experiments led to the assumption that Azoarcus doesn`t use an AHL based quorum sensing system, but probably uses a novel autoinducer (HSF, hydrophilic signal factor) for intercellular communication.
Keywords: type IV pili, twitching motility, quorum sensing, autoinducer
Issue Date: 11-Jan-2007
Type: Dissertation
URN: urn:nbn:de:gbv:46-diss000106092
Institution: Universität Bremen 
Faculty: FB2 Biologie/Chemie 
Appears in Collections:Dissertationen

  

Page view(s)

24
checked on Jan 25, 2021

Download(s)

35
checked on Jan 25, 2021

Google ScholarTM

Check


Items in Media are protected by copyright, with all rights reserved, unless otherwise indicated.

Legal notice -Feedback -Data privacy
Media - Extension maintained and optimized by Logo 4SCIENCE