Triggered release of liposome contents by the divalent anion B12H11SH(2-): Biochemical studies

Abstract

BSH is one of the two boron compounds in use in BNCT. Triggered release of liposome contents by BSH has been achieved. We used liposomes of different compositions as a model for studying the interaction of BSH with cell membranes. The interaction of BSH with cationic liposomes of different compositions was investigated. Zeta potential and size measurements of the liposomes in absence and presence of BSH indicated a firm binding of BSH with the headgroups of these liposomes. Beside exploiting the liposomes as model for cell membranes, there is also great interest in liposomes as drug carriers. To have a pronounced therapeutic effect, the release of the drug from the liposomes should be as complete as possible. Because of this, developing tools for triggering release of agents encapsulated inside the aqueous compartment of the liposomes is an important factor in the liposome drug delivery systems.We studied the interaction of BSH with neutral liposomes prepared either from DMPC or DPPC lipids and proved that they released their contents under the influence of BSH within minutes.