Skip navigation
SuUB logo
DSpace logo

  • Home
  • Institutions
    • University of Bremen
    • City University of Applied Sciences
    • Bremerhaven University of Applied Sciences
  • Sign on to:
    • My Media
    • Receive email
      updates
    • Edit Account details

Citation link: http://nbn-resolving.de/urn:nbn:de:gbv:46-diss000103530
00010353.pdf
OpenAccess
 
copyright

Evaluation of the Effects of Tyrosine Kinase Inhibitors on the Metabolism of Human Tumor Cells using an NMR-based Biochemical Profiling Strategy


File Description SizeFormat
00010353.pdf1.85 MBAdobe PDFView/Open
Other Titles: Untersuchung der Effekte von Tyrosinkinase Hemmern auf den Metabolismus humaner Tumorzellen mit Hilfe von NMR-Spektroskopie und anderen biochemischen Methoden
Authors: Miljus, Jelena 
Supervisor: Leibfritz, Dieter
1. Expert: Leibfritz, Dieter
2. Expert: Christians, Uwe
Abstract: 
Imatinib (Gleevec) is one of the first molecularly targeted cancer therapy drugs, successfully approved for the treatment of chronic myelogenous leukemia. The goal of this project was to assess the metabolic profiles and to gain mechanistic insights into the effects of tyrosine kinase inhibitors on cell metabolism. Most studies were based on nuclear magnetic resonance and mass spectrometry metabolic profiling. Treatment with imatinib induced apoptosis in human leukemia cells. Different stages of apoptotic cell death have been distinguished showing specific metabolic patterns. Imatinib resistant cell lines have shown characteristic profiles in regards to their glycolysis and lipid metabolism. After imatinib withdrawal, resistant cells underwent apoptosis and were regaining some of the properties of their parental clones. These findings may be important in the evaluation, identification, prediction of patients developing resistance and treatment of cancer. Based on these results, metabolic profiling of blood from imatinib-treated patients has the potential to be developed into a diagnostic tool to detect the imatinib resistance at an early stage.
Keywords: Imatinib mesylate, Gleevec, ZD1839, Iressa, 5-Fluorouracil, Celecoxib, chronic myelogenous leukemia, CML, nuclear magnetic resonance, NMR, tyrosine kinase inhibitors, apoptosis, drug resistance
Issue Date: 17-May-2006
Type: Dissertation
URN: urn:nbn:de:gbv:46-diss000103530
Institution: Universität Bremen 
Faculty: FB2 Biologie/Chemie 
Appears in Collections:Dissertationen

  

Page view(s)

21
checked on Feb 25, 2021

Download(s)

6
checked on Feb 25, 2021

Google ScholarTM

Check


Items in Media are protected by copyright, with all rights reserved, unless otherwise indicated.

Legal notice -Feedback -Data privacy
Media - Extension maintained and optimized by Logo 4SCIENCE