Skip navigation
SuUB logo
DSpace logo

  • Home
  • Institutions
    • University of Bremen
    • City University of Applied Sciences
    • Bremerhaven University of Applied Sciences
  • Sign on to:
    • My Media
    • Receive email
      updates
    • Edit Account details

Citation link: http://nbn-resolving.de/urn:nbn:de:gbv:46-diss000006903
E-Diss690_ahmann.pdf
OpenAccess
 
copyright

In vitro Studie zur neurotrophen Wirkung des Augenlinsen-Proteins BetaH-Kristallin auf Motoneurone des embryonalen Hühnchens (Gallus gallus)


File SizeFormat
E-Diss690_ahmann.pdf9.52 MBAdobe PDFView/Open
Authors: Ahmann, Petra 
Supervisor: Kreiter, Andreas
1. Expert: Kreiter, Andreas, Thanos, Solon 
Abstract: 
AbstractCrystallins are water soluble proteins expressed abundantly in the eye lens, where their cell-protective function is known. In the first part of this study, it was examined whether different HPLC-fractions of lenticular crystallins exert an increase of neurite outgrowth in the organotypically cultured spinal cord. Next, different concentrations of HPLC-fractionated lens proteins from the rat were compared to the neurotrophic factors NGF, CNTF and NT-4. It was shown here that all mammalian crystallins promote an increase in neurite outgrowth of spinal neurites in vitro. The a- and bH-crystallin fractions had the greatest maximal neurotrophic effect of all the crystallins and their effect was comparable to NGF and NT-4 in organotypic chick spinal cord culture. In the second part of this study the bH-crystallin was tested on dissociated spinal motor neurons. bH-crystallin promoted a direct increase of neurite length in motor neurons without involvement of microglia or macrophages. In the third part of this study it was determined if bB1-crystallin, a dominant subunit of bH-crystallin, showed a similar effect. Motor neurons in single cell culture were treated with recombinant human wildtype bB1-crystallin and it was found, that wildtype bB1-crystallin alone exerted the same growth promoting effect as the whole bH-crystallin fraction.
Keywords: crystallin; neurite outgrowth; neurite length, in vitro; motor neurons, spinal cord
Issue Date: 20-Nov-2003
Type: Dissertation
URN: urn:nbn:de:gbv:46-diss000006903
Institution: Universität Bremen 
Faculty: FB2 Biologie/Chemie 
Appears in Collections:Dissertationen

  

Page view(s)

18
checked on Jan 22, 2021

Download(s)

16
checked on Jan 22, 2021

Google ScholarTM

Check


Items in Media are protected by copyright, with all rights reserved, unless otherwise indicated.

Legal notice -Feedback -Data privacy
Media - Extension maintained and optimized by Logo 4SCIENCE