Skip navigation
SuUB logo
DSpace logo

  • Home
  • Institutions
    • University of Bremen
    • City University of Applied Sciences
    • Bremerhaven University of Applied Sciences
  • Sign on to:
    • My Media
    • Receive email
      updates
    • Edit Account details

Citation link: http://nbn-resolving.de/urn:nbn:de:gbv:46-diss000006071
E-Diss607_Diss_Dobrinski.pdf
OpenAccess
 
copyright

Entwicklung und Charakterisierung von siliziumbasierten mikrotechnischen Komponenten für miniaturisierte DNA-Analysen


File SizeFormat
E-Diss607_Diss_Dobrinski.pdf8.65 MBAdobe PDFView/Open
Other Titles: Development and characterization of silicon-based microfabricated components for miniaturized DNA analyses
Authors: Dobrinski, Heiko 
Supervisor: Benecke, Wolfgang
1. Expert: Benecke, Wolfgang
2. Expert: Silber, Dieter
Abstract: 
This thesis deals with the design, fabrication and characterization of microsystems for use as single components within a miniaturized multistage DNA analysis. Within the first part of this work the example of a new microreaction chamber is used to demonstrate the suitability of silicon-based microsystems for DNA amplification by the temperature- and enzymatically controlled polymerase chain reaction (PCR). Covering design and fabrication aspects, two alternative system concepts are presented and compared. By the selected microchamber with double-sided heaters and sensor elements for temperature-controlling a high temperature homogeneity as well as maximum heating and cooling rates are achieved. A main focus of the characterization is the analysis of binding reactions of single-stranded oligonucleotide primers, double-stranded template molecules and the polymerase on microchamber surfaces. The successful DNA amplification in microchambers with volumes of 6 µl are demonstrated, whereas for a complete PCR with 30 cycles less than 18 min are needed.The second part is focused on a novel measurement principle for real-time detection of hybridization reactions on planar sensor surfaces by an impedance analysis of metallicinterdigitated electrodes. By using functionalized polymer microparticles, so-called microbeads, for labeling of DNA to be analyzed an increasing of the signal intensity is achieved.For modeling the sensor behaviour a two-dimensional electrical equivalent circuit is developed and applied for deriving of design and measurement criterions. The fabricated single sensor element consists of a thin film interdigitated electrode based on a tungsten titanium alloy with a dielectric passivation layer. Monocrystalline silicon and amorphous quartz glass are used as substrate materials. By experiments in a stopped-flow hybridization buffer the proof of functionality of the sensor element is provided.
Keywords: Mikrosystemtechnik, Siliziumtechnologie, Polymerasekettenreaktion, Hybridisierung, DNA
Issue Date: 16-Jul-2003
Type: Dissertation
URN: urn:nbn:de:gbv:46-diss000006071
Institution: Universität Bremen 
Faculty: FB1 Physik/Elektrotechnik 
Appears in Collections:Dissertationen

  

Page view(s)

18
checked on Jan 19, 2021

Download(s)

4
checked on Jan 19, 2021

Google ScholarTM

Check


Items in Media are protected by copyright, with all rights reserved, unless otherwise indicated.

Legal notice -Feedback -Data privacy
Media - Extension maintained and optimized by Logo 4SCIENCE