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  4. Photosynthesis in Antarctic sea-ice diatoms
 
Zitierlink URN
https://nbn-resolving.de/urn:nbn:de:gbv:46-diss000005663

Photosynthesis in Antarctic sea-ice diatoms

Veröffentlichungsdatum
2003-05-09
Autoren
Mock, Thomas  
Betreuer
Smetacek, Victor  
Gutachter
Kirst, Gunter Otto  
Zusammenfassung
A systematic approach of investigations was applied to obtain precise measurements of photosynthesis under natural conditions in the field from which questions were derived for further analysis in the laboratory. In situ measurements with the tracer 14C through the entire thickness of a young sea ice floe revealed that algae are able to actively assimilate dissolved inorganic carbon under extreme conditions after inclusion into newly formed sea ice. These measurements were conducted with ice slices suspended in a new incubator. New sensors (oxygen micro-optodes) were introduced into sea ice research which withstand freezing without damage. A new laboratory sea ice microcosm was developed to simulate natural conditions of sea ice and to cultivating a sea-ice diatom (Fragilariopsis cylindrus) directly within brine channels. However, how changes of environmental conditions influence photosynthesis and how these diatoms are generally adapted to their habitat still remained unresolved. Consequently, the first molecular data base of an obligate psychrophilic diatom (Fragilariopis cylindrus) was conducted using an EST (expressed sequence tag) approach under freezing conditions. More than half (59%) of these sequences could not be identified by GenBank comparison, indicating the existence of many unknown genes. A subset of identified genes and additional genes responsible for photosynthesis, respiration and cold adaptation were cloned and arranged on a macro-array to investigate gene expression under freezing conditions. These molecular measurements were paralleled by biophysical and biochemical investigations. Experimental results revealed that acclimation of diatom photosynthesis under freezing temperatures of sea water and different resource limitations (light, nutrients) requires chaperons and repair mechanisms in order to sustain chloroplast membranes and proteins which are responsible for energy generation and carbon dioxide fixation.
Schlagwörter
photosynthesis

; 

diatom

; 

sea ice

; 

Fragilariopsis cylindrus

; 

biooptics

; 

cold adaptation

; 

gene expression

; 

EST-library
Institution
Universität Bremen  
Fachbereich
Fachbereich 02: Biologie/Chemie (FB 02)  
Dokumenttyp
Dissertation
Zweitveröffentlichung
Nein
Sprache
Englisch
Dateien
Lade...
Vorschaubild
Name

E-Diss566_Mock.pdf

Size

6.38 MB

Format

Adobe PDF

Checksum

(MD5):e2c487d487ccae36169c7d051098bfd0

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