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  4. Quantifizierung von Strukturen, Kinetik und Dynamik nekrobiologischer Prozesse mit Hilfe bildverarbeitender konfokaler Fluoreszenzmikroskopie
 
Zitierlink URN
https://nbn-resolving.de/urn:nbn:de:gbv:46-diss000000561

Quantifizierung von Strukturen, Kinetik und Dynamik nekrobiologischer Prozesse mit Hilfe bildverarbeitender konfokaler Fluoreszenzmikroskopie

Veröffentlichungsdatum
2000-04-11
Autoren
Härtel, Steffen  
Betreuer
Diehl, Horst  
Gutachter
Gabel, Detlef  
Zusammenfassung
Translocation of phosphatidylserine (PS) from the inner to the outer plasma membrane leaflet is presently discussed as a hallmark of apoptosis. The signalling which initialises the process and the translocation mechanism itself are still under investigation. Diffusion or enzymatic transport is suspected. Staurosporine induced PS-translocation was monitored in HCE-cells and in mouse lymphocytes (EL-4) by Annexin V-FITC (AV) fluorescence. QFM could not only reveal the percentage of AV positive and AV negative cells in both cultures, it could also derive that once initiated, PS-translocation in single HCE-cells is completed within less than ½h. This and other observations support nondiffusive PS-translocation. For toxicological investigations, early apoptotic parameters are essential. QFM detected two very early staurosporine induced effects in HCE- and EL-4-cells. In HCE-cells, a morphologic form of Karyorrhexis was discovered as early as 10min after induction. This form of chromatin condensation is probably initiated by the mitochondrial apoptotic inducing factor (AIF), which was recently discovered. Activated caspase-3, which is also discussed to initialise PS-translocation and chromatin condensation could be excluded for both events in HCE-cells. In EL-4-cells, perturbation of cellular migration and the exclusion of small cellular vesicles were detected within minutes after staurosporine induction. The results presented in this and other scientific publications validate the OFM method as a promising tool for future investigations. The method should work on many cellular events, in which fluorescent or non fluorescent texture or morphological features must be connected with statistical information.
Schlagwörter
el-4 ipc-81 hce image processing confocal microscopy fluorescent membrane dyes chromatin condensation DiI Laurdan DPH Syto13 segmentation scaling indices SIM
Institution
Universität Bremen  
Fachbereich
Fachbereich 01: Physik/Elektrotechnik (FB 01)  
Dokumenttyp
Dissertation
Zweitveröffentlichung
Nein
Sprache
Deutsch
Dateien
Lade...
Vorschaubild
Name

E-Diss56_Haertel_S2000.pdf

Size

10.25 MB

Format

Adobe PDF

Checksum

(MD5):cf15a1d2e4ed90c9e71aac9f00388711

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