Rabus, Ralf
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Rabus, Ralf
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Rabus, Ralf
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Item type:Publication, Proteomische und genetische Untersuchungen zum Aromaten-Abbau in "Aromatoleum aromaticum" Stamm EbN1(2008-06-12); ; ; Aromatic compounds are widely distributed and abundant in nature being main constituents of crude oil and important products of higher plants. The denitrifying Aromatoleum aromaticum strain EbN1 utilizes a wide range of aromatic and nonaromatic compounds under anoxic and oxic conditions. The recently determined genome sequence revealed corresponding degradation pathways and predicted a fine-tuned regulatory network.This work reports on the analysis of substrate specific regulation of anaerobic and aerobic degradation pathways in "A. aromaticum" strain EbN1 grown with 17 different aromatic and aliphatic compounds under oxic and anoxic conditions applying differential proteomics (2D DIGE). The comprehensive approach enabled the discovery of so far unaccounted catabolic capacities and their subsequent elucidation on the physiological, molecular and regulatory level. The adaptation of strain EbN1 to solvent stress was investigated on the physiological and proteomic level since strain EbN1 is able to degrade several toxic alkylbenzenes and alkylphenols. In addition, a genetic system for strain EbN1 is described enabling unmarked deletion mutagenesis.doctoral thesis254 417 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Metabolische Regulation und Rekonstruktion bei :i: Desulfobacterium autotrophicum :/i:(2004-12-22); ; ; :i: Desulfobacterium autotrophicum :/i: is a marine sulfate-reducing bacterium, which is metabolically quite versatile. Here the it was tried to investigate metabolic regulation using a physiological, proteomic and RNA based methods. The investigations were focussed on cold-adaption, alcohol metabolism and the terminal oxidation of substrates.doctoral thesis267 92 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Regulation of anaerobic catabolism of aromatic compounds and sulfate reduction in Desulfobacula toluolica Tol2(2007-09-20); ; ; The metabolic regulation of the aromatic-compounds degrading sulfate-reducing bacteria Desulfobacula toluolica Tol2 in response to the availability and adaptation to aromatic growth substrates (toluene, phenylacetate, p-cresol, p-hydroxybenzaldehyde, p-hydroxybenzoate and benzoate) was studied by a combined physiological/proteomic approach. Also, regulation of key subunits of dissimilatory sulfate reduction pathway (adenosine-5'-phosphosulfate reductase subunit A, dissimilatory sulfite reductase subunit A and dissimilatory sulfite reductase subunit B) was studied under different electron-acceptor conditions (growth with sulfate, sulfite, thiosulfate or a mixture of sulfate, sulfite and thiosulfate) utilizing benzoate as aromatic growth substrate.doctoral thesis390 108 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Untersuchungen zur Regulation der anaeroben Verwertung von Alkylbenzolen im denitrifizierenden Stamm EbN1(2005-12-09); ; ; The anaerobic degradation pathways of toluene and ethylbenzene and the adaptation to toxicological effects of these alkylbenzenes are studied with the denitrifying strain EbN1 in a combined physiological and proteomic approach. How specific is the substrate-dependend regulation of the two degradation pathways? Which molecular-physiological effects become apparent at growth with sublethal concentrations? What reaction follows an alkylbenzene-shock in absence of operative degradation pathways?doctoral thesis215 167