Veltel, StefanStefanVeltelMoreno-Layseca, PaulinaPaulinaMoreno-LaysecaJäntti, NiklasNiklasJänttiGodbole, RashmiRashmiGodboleSommer, ChristianChristianSommerJacquemet, GuillaumeGuillaumeJacquemetAl-Akhrass, HusseinHusseinAl-AkhrassConway, James R. W.James R. W.ConwayKronqvist, PauliinaPauliinaKronqvistKallionpää, Roosa E.Roosa E.KallionpääOliveira-Ferrer, LeticiaLeticiaOliveira-FerrerCervero, PasqualePasqualeCerveroLinder, StefanStefanLinderAepfelbacher, MartinMartinAepfelbacherZauber, HenrikHenrikZauberRae, JamesJamesRaeParton, Robert G.Robert G.PartonDisanza, AndreaAndreaDisanzaScita, GiorgioGiorgioScitaMayor, SatyajitSatyajitMayorSelbach, MatthiasMatthiasSelbachIvaska, JohannaJohannaIvaska2023-01-122023-01-1220211465-7392https://media.suub.uni-bremen.de/handle/elib/6449Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery—Arf1, IRSp53 and actin—and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.enAlle Rechte vorbehaltenbreast cancercell adhesionendosomes500 Naturwissenschaften und Mathematik::570 Biowissenschaften; BiologieText::Zeitschrift::Wissenschaftlicher Artikel