Purification, identification, and recombinant expression of peroxidases from marine macroalgae
Veröffentlichungsdatum
2025-09-17
Autoren
Betreuer
Gutachter
Kolkenbrock, Stephan
Zusammenfassung
In this study investigates peroxidases from Baltic Sea macroalgae with regard to their identification, recombinant production and biotechnological potential. In contrast to well-studied plant peroxidases, algal peroxidases remain largly unexplored, particularly with respect to their function. Transcriptomic data, native protein purification, and mass spectrometry were combined to identify peroxidases from the macroalgae. Three candidates were selected for heterologous expression in E. coli. Two candidates were successfully produced and biochemically characterized following cofactor reconstitution. A cytochrome c peroxidase from the green alga Bryopsis plumosa was analyzed with respect to activity and stability, while a vanadium-dependent haloperoxidase from the red alga Ahnfeltia plicata, including a truncated variant, was applied in a luminol-based chemiluminescence assay.
Schlagwörter
marine biotechnology
;
peroxidases
;
transcriptomics
;
recombinant expression
;
macroalgae
;
mass spectrometry
;
haloperoxidase
;
chemiluminescence
Institution
Fachbereich
Dokumenttyp
Dissertation
Sprache
Englisch
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Purification, identification, and recombinant expression of peroxidases from marine macroalgae.pdf
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