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  4. Polyphasische Taxonomie, Kerngenom und Lebenszyklus von Rhodopirellula-Stämmen
 
Zitierlink URN
https://nbn-resolving.de/urn:nbn:de:gbv:46-00102198-11

Polyphasische Taxonomie, Kerngenom und Lebenszyklus von Rhodopirellula-Stämmen

Veröffentlichungsdatum
2011-07-04
Autoren
Frank, Carsten S.  
Betreuer
Harder, Jens  
Gutachter
Amann, Rudolf  
Zusammenfassung
This thesis focused on three major projects dealing with the planctomycetes of the species Rhodopirellula baltica. In a polyphasic taxonomic study the morphological, physiological and chemotaxonomic characteristics of 17 newly isolated strains were investigated. Based on the obtained results and phylogenetic data ten new Rhodopirellula species were introduced along with one new genus. The physiology of the strains was studied with regard to the influence of ammonium on their life style. Using flow cytometry an increase of aggregated cells compared to single cells was observed parallel to an increase of the ammonium concentration. In chemostat cultures an ammonium shock induced the formation of a R. baltica biofilm by attachment of cells to the fermenter wall. With ten strains the Rhodopirellula core genome was determined by microarray analysis and genome sequencing. Both methods were compared. The R. baltica core genome contains 3314 shared genes according to microarray analysis and 4232 shared genes according to genome annotation. The investigations of this work are the basis for future comparative studies between Rhodopirellula species.
Schlagwörter
Rhodopirellula baltica

; 

Planctomycetales

; 

ammonium

; 

chemostat

; 

flow cytometry

; 

Carbon/nitrogen ratio

; 

bioflim

; 

polyphasic taxonomy

; 

chemotaxonomy

; 

microarray

; 

whole genome analysis

; 

comparative genomics
Institution
Universität Bremen  
Fachbereich
Fachbereich 02: Biologie/Chemie (FB 02)  
Dokumenttyp
Dissertation
Zweitveröffentlichung
Nein
Sprache
Deutsch
Dateien
Lade...
Vorschaubild
Name

00102198-1.pdf

Size

14.49 MB

Format

Adobe PDF

Checksum

(MD5):f1cd118d00ea6d96ffb4740c493bd7e1

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