Bedeutung von SWEET-Genen für den pflanzlichen Zuckerexport in einer Ektomykorrhizasymbiose
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
00105866-1.pdf | 19.56 MB | Adobe PDF | Anzeigen |
Sonstige Titel: | Impact of SWEET genes on host plant sugar export in ectomycorrhizal symbiosis | Autor/Autorin: | Neb, Dimitri ![]() |
BetreuerIn: | Nehls, Uwe | 1. GutachterIn: | Nehls, Uwe | Weitere Gutachter:innen: | Reinhold-Hurek, Barbara | Zusammenfassung: | In mutualsitic interactions between fungi and plants, such as ectomycorrhiza, the fungus contributes nutrition and obtains plant derived carbohydrates as a response. The carbohydrate transport in ectomycorrhized roots is not yet fully understood and requires additional investigations. However prerequisites for such analysis are transgenic plants. Poplar is a well established model for investigation of ectomycorrhizal symbiosis. As the generation of transgenic poplar plants is a highly time consuming process and can take up to one year a new A. rhizogenes- based transformation protocol was developed in the present thesis. Furthermore, the role of the recently discovered SWEET-proteins in the carbohydrate transport in ectomycorrhiza was investigated. Ectomycorrhiza regulated SWEET proteins were localized in the plasma membrane of Nicotiana benthamiana leaves determining their role in the carbohydrate supply of the fungal partner in ectomycorrhiza. Additionally a promoter analysis of one of the mycorrhiza-induced SWEET genes, PtSWEET 1.c, was performed and two regulatory regions could be identified. However, an ectomycorrhiza specific regulation of the promoter reporter constructs was not observed. |
Schlagwort: | Ektomykorrhiza; Komposit-Pappeln; Promotoranalyse; Zuckertransport; Wurzel Transformation | Veröffentlichungsdatum: | 10-Mär-2017 | Dokumenttyp: | Dissertation | Zweitveröffentlichung: | no | URN: | urn:nbn:de:gbv:46-00105866-16 | Institution: | Universität Bremen | Fachbereich: | Fachbereich 02: Biologie/Chemie (FB 02) |
Enthalten in den Sammlungen: | Dissertationen |
Seitenansichten
294
checked on 02.04.2025
Download(s)
228
checked on 02.04.2025
Google ScholarTM
Prüfe
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.