Medlin, Linda K.

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Medlin, Linda K.
Official Name
Medlin, Linda K.
Alternative Name
Medlin, Linda Karen
Medlin, L. K.
Medlin, Linda
Medlin, L.
ORCID
GND

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    Item type:Publication,
    Development and evaluation of rapid and semi-automated devices for the detection of toxic algae
    (2007-04-02)
    Diercks, Sonja
    ;
    Cembella, Allan
    ;
    Kirst, Gunter
    ;
    Medlin, Linda
    A commercially available PCR ELISA Dig Detection Kit was successfully adapted for the detection of the toxic dinoflagellate Alexandrium minutum by application of ribosomal 18S probes and sandwich hybridization. Furthermore, the assay showed the specificity of 10 additional probe sets for the toxic algal species. The total rRNA isolation protocol was improved and the hybridization procedure for the electrochemical detection of toxic algal species was described in detail and visualized for easy application by inexperienced users. Furthermore, locked nucleic acid probes were tested with known target sequences and the specificity and sensitivity was compared to signal formation of conventional molecular probes. The hybridization signals for both tested solid surface methods could not be enhanced and the conventional DNA probes showed equal or better results. A stand-alone, semi-automated system in combination with multiprobe chips was developed. A multiprobe chip with 16 gold electrodes was designed and adapted for the use in a sandwich hybridisation assay.
    doctoral thesis
      187  92
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    Estimation of genetic diversity in the colony forming polar prymnesiophyte species Phaeocystis antarctica
    (2009-11-30)
    Gäbler-Schwarz, Steffi
    ;
    Kirst, Gunter O.
    ;
    Bathmann, Ulrich
    ;
    Medlin, Linda K.
    This thesis provides a detailed insight into genetic diversity among and between Phaeocystis antarctica populations from different regions of the Southern Ocean and the gene flow between them. Genetic, physiological and ecological results are discussed in a broader view giving valuable insights into the ecology of this keystone species in the Southern Ocean. This work is opening a new field of research on P. antarctica, which can be termed 'ecophysiological genetics'. By combining genetic data with results from ecological investigations and physiological experiments, a broader understanding of ecosystem functioning will also direct future studies.
    doctoral thesis
      304  170